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phenotype from the PAN66 mutant strain was complemented by the introduction of the distinctive gene. The dearth of attachment in the mutant PAN66 was consequently thanks exclusively into the disruption of your ladN66
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, The most crucial operate from the flagellum is just not in adhesion, but in facilitating bacterial motility.
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adhesins, connected to the adhesins in other microorganisms (14). We confirmed that mutants devoid of a practical CupA are faulty while in the formation of biofilm, inside a fashion that's impartial on the existence of kind IV pili. Utilizing the available sequence in the PAO1 genome, we identified further cup
The bacterial strains and plasmids utilised are stated in Desk Table1.one. Strains have been developed at 37°C in L broth (LB) or on LB agar plates. Plasmids were introduced into P. aeruginosa
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The DNA sequences received had been analyzed With all the blastn program and when compared With all the PAO1 genome at . The putative ORFs have been then analyzed with ORF finder (). The annotated genome was employed on launch to update our analysis.
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